Henderson Police Chief LaTesha Watson was placed on administrative leave by the city earlier Thursday.
Deputy Police Chief Thedrick Andres will serve as acting police chief until further notice.
A city spokeswoman did not give a reason for the action.
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Watson was first hired as chief of police in November of 2017 after the ouster of Patrick Moers amid allegations of sexual harassment.
Last November, two police unions filed a complaint against Watson with the Employee-Management Relations Board.